DNA Extraction Control enables users of diagnostic assays to validate their extraction step. DNA Extraction Control contains a known concentration of cells that contain the control DNA sequence. Cells containing internal control DNA are spiked into the lysis buffer with the sample prior to DNA extraction.
Following DNA extraction, the reaction mix is added to the extracted DNA prior to amplification. All components required for amplification of sample DNA should also be added. Presence of internal control DNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample DNA.