RNA Extraction Control enables users of diagnostic assays to validate their extraction step. RNA Extraction Control contains a known concentration of artificial cells possessing the control RNA sequence. Cells containing internal control RNA are “spiked” into the lysis buffer with the sample prior to RNA extraction.
Following extraction the reaction mix is added to the extracted RNA prior to reverse transcription and amplification. Presence of internal control RNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample RNA.