SensiFAST Lyo-Ready No-Rox Mix, 2x, 10 x 10 mL

Product Highlights
Glycerol – free – ideal for preparation of custom lyophilized qPCR master mixes for improved convenience and extended room-temperature stability
Reproducible – consistent results between technical replicates for increased confidence in results
Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types
Sensitive – reliable quantification of low abundance targets and scarce samples
Fast – delivers reproducible, accurate assay results in as little as 30 minutes
Efficient – excellent performance in multiplex assays
Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability

Product Description
The SensiFAST™ Lyo-Ready No-ROX Mix is a glycerol-free qPCR mix that has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.

A combination of the latest advances in buffer chemistry, PCR enhancers and lyo-excipients, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Lyo-Ready No-ROX Mix produces reliable assay results under fast thermal cycling conditions and when multiplexing of high- and low-copy targets.

SensiFAST Lyo-Ready No-ROX Mix together with assay-specific primers and probes can be lyophilized to produce ambient temperature stable qPCR master mixes. Millisecond rehydration times make SensiFAST Lyo-Ready No-ROX Mix ideal for automated, high throughput systems.

Applications
Diagnostic test development
Pathogen detection
Drug therapy efficacy
DNA / RNA target detection
Copy number variation (CNV) analysis
Gene expression analysis

Resources

SensiFAST qPCR Guide Fact Sheet