MyTaq Mix, 2x, 1000 Reactions

Product Highlights

Sensitive – exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
Convenient – an all-in-one-tube mastermix that improves the speed, convenience and accuracy of PCR set-up
Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions

Product Description

MyTaq™ Mix is recommended for all standard PCR applications. MyTaq Mix a unique combination of MyTaq DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq means it gives excellent results under fast PCR conditions.

The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl2 and enhancers at optimal concentrations helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

The combination of MyTaq and optimized buffer system allow for faster PCR reactions compared with other polymerases, therefore reducing overall run time from approximately 1 hour to under 30 minutes. This is achieved without compromising specificity or yield, reducing the reaction time allows for increased throughput and faster time to results.

Applications

Standard PCR
High-yield PCR
Fast PCR
Colony PCR
TA cloning

Resources

MyTaq DNA Polymerase and Mixes Fact Sheet