Long oligonucleotide -more thermostable Reverse Transcriptases perform better with longer primers
Binds only to mRNA -can be used for cDNA synthesis with total RNA
Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented.
Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´
A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.
cDNA synthesis with a reverse transcriptase