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Real-Time PCR Kits

SensiFAST Probe No-ROX Kit 5000 rxns

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.

SKU: BIO-86050 Category:

Product Highlights
Reproducible – consistent results between technical replicates for increased confidence in results
Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
Sensitive – reliable quantification of low abundance targets and scarce samples
Fast – delivers reproducible, accurate assay results in as little as 30 minutes
Efficient – excellent performance in multiplex assays
Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability

Product Description
The SensiFAST™ Probe No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.

A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays.

The SensiFAST Probe No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications
Gene expression analysis
DNA / RNA target detection
Copy number variation (CNV) analysis



Resources

SensiFAST qPCR Guide Fact Sheet

Additional information

Item Number

BIO-86020

Brand

Bioline