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Tetro Reverse Transcriptase Enzyme , 10,000 Units

SKU: BIO-65050 Category:

Product Highlights
Highly sensitive – for high-quality, full length cDNA from as little as 10 pg of total RNA
Ultra-stable – for long genes and rare transcripts
High yield – produces high quality cDNA ideal for PCR
Broad dynamic range – 10 pg to 5 μg of RNA

Product Description
Tetro™ Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (fig. 1), with highly efficient and sensitive transcription, from as little as 10 pg, up to 5 μg of RNA (fig. 2).

Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures.

Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.


Resources 

Tetro Reserve Transcriptase and cDNA Synthesis Kit

Additional information

Item Number

BIO-65051

Brand

Bioline