Accurate – clear discrimination of even the most challenging sequence differences
Reproducible – unparalleled consistency between replicate difference plots for increased confidence in sample characterization
Sensitive – efficient detection from even very limiting amounts of sample
Flexible – enables reliable characterization of a broad range of sequence differences
Fast – rapid PCR amplification prior to high resolution melting, enabling higher throughput
SensiFAST HRM Kit has been developed for detailed characterization of samples according to their base composition, length and GC content by high resolution melting. The latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensure the SensiFAST HRM Kit delivers reproducible, accurate HRM analysis.
SensiFAST HRM Kit contains EvaGreen®, a third generation, saturating fluorescent dye which selectively binds to double-stranded DNA. In contrast to dyes such as SYBR® Green I, EvaGreen can be used at higher concentrations without inhibiting PCR and shows equal binding affinity for GC-rich and AT-rich regions. The combination of our SensiFAST DNA Polymerase, unique buffer system and EvaGreen dye enables amplification and discrimination of even the most challenging sequence differences, such as class IV SNPs without sequence preference. Since it does not require expensive labelled oligonucleotide probes, SensiFAST HRM Kit is also a cost effective alternative to traditional probe based genotyping methods.
Characterization of haplotype blocks
Detection of insertions, deletions and translocations
DNA methylation analysis
Allelic prevalence population studies
Screening for loss of heterozygosity