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Real-Time PCR Kits

SensiFAST SYBR Lo-ROX One-Step Kit , 100 rxns

Sensitive, reproducible detection of even the most challenging RNA targets, under fast thermal cycling conditions.

SKU: BIO-74001 Category:

Product Highlights
Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
Reproducible – consistent results between technical replicates for increased confidence in results
Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
Robust – reliable detection of RNA targets from a broad range of sample types
Fast – delivers reproducible, accurate assay results in as little as 40 minutes

Product Description
The SensiFAST SYBR® Lo-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube.

An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.

The SensiFAST SYBR® Lo-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX.

Applications
Gene expression analysis
Pathogen detection
RNA viral pathogen detection
Genetic profiling
miRNA profiling / quantification
Genetically modified organisms (GMO) characterization



Resources

SensiFAST qPCR Guide Fact Sheet

Additional information

Item Number

BIO-74001

Brand

Bioline